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Daily Cardiology Research Analysis

3 papers

Three impactful cardiology studies stood out: (1) flexible mesh nanoelectronics revealed arrhythmogenic automaticity in transplanted human iPSC-derived cardiomyocytes and a self-assembling peptide (RADA16) mitigated it; (2) intravascular imaging guidance during PCI of severely calcified lesions was associated with lower 1-year target vessel failure versus angiography alone; (3) neutrophil-to-lymphocyte ratio (≥4) provided robust risk stratification in myocarditis, especially with preserved LVEF.

Summary

Three impactful cardiology studies stood out: (1) flexible mesh nanoelectronics revealed arrhythmogenic automaticity in transplanted human iPSC-derived cardiomyocytes and a self-assembling peptide (RADA16) mitigated it; (2) intravascular imaging guidance during PCI of severely calcified lesions was associated with lower 1-year target vessel failure versus angiography alone; (3) neutrophil-to-lymphocyte ratio (≥4) provided robust risk stratification in myocarditis, especially with preserved LVEF.

Research Themes

  • Mechanistic safety insights for cardiac regeneration using nanoelectronics and biomaterials
  • Precision interventional cardiology with intravascular imaging guidance in calcified PCI
  • Inflammation-based risk stratification in myocarditis across the LVEF spectrum

Selected Articles

1. Flexible nanoelectronics reveal arrhythmogenesis in transplanted human cardiomyocytes.

79Level VCase seriesScience (New York, N.Y.) · 2025PMID: 41100583

Using flexible mesh nanoelectronics in beating rat hearts, the authors directly recorded fibrillation and automaticity from transplanted human iPSC-derived cardiomyocytes. The self-assembling peptide RADA16 promoted maturation and vascularization while markedly suppressing arrhythmogenic automaticity, highlighting a combined bioelectronic–biomaterial strategy to improve cardiac cell therapy safety.

Impact: This study introduces an in vivo nanoelectronic platform to interrogate and mitigate arrhythmias from grafted human cardiomyocytes, addressing a key safety barrier in cardiac cell therapy. The mechanistic insight and a clinically approved peptide intervention offer a translational path.

Clinical Implications: Highlights an approach to reduce graft-related arrhythmias by conditioning the graft microenvironment (RADA16) and monitoring with implantable nanoelectronics. May inform peri-transplant strategies and safety endpoints for human cardiac cell therapy trials.

Key Findings

  • Flexible mesh nanoelectronics detected fibrillation and spontaneous activity from transplanted hiPSC-CMs in vivo.
  • RADA16 accelerated adult-like gene expression, enhanced sarcomere organization, and improved vascularization at the graft site.
  • RADA16 markedly reduced arrhythmogenic automaticity in transplanted hiPSC-CMs.

Methodological Strengths

  • Innovative in vivo electrophysiological mapping with flexible mesh nanoelectronics in a beating heart.
  • Multimodal assessment (transcriptomic maturation, myofibrillar structure, vascularization) with a clinically approved biomaterial.

Limitations

  • Preclinical animal model; human clinical efficacy and long-term safety remain untested.
  • Quantitative arrhythmia burden and durability beyond the study window were not fully detailed.

Future Directions: Validate nanoelectronic monitoring and RADA16 conditioning in large-animal models, define dosing/timing, and develop clinical-grade sensors to monitor graft electrophysiology in first-in-human trials.

2. Intravascular Imaging vs Angiography Guidance for PCI of Severely Calcified Lesions: The ECLIPSE Trial.

77Level IIICohortJACC. Cardiovascular interventions · 2025PMID: 41093451

In this large randomized trial cohort of severely calcified lesions, intravascular imaging guidance (OCT/IVUS) was associated with a significantly lower 1-year target vessel failure versus angiography alone (adjusted HR 0.74). Benefits were consistent irrespective of vessel preparation strategy (orbital atherectomy or balloon angioplasty).

Impact: Demonstrates outcome benefit of IVI guidance in a challenging real-world population (severely calcified lesions), informing procedural standards and training priorities.

Clinical Implications: Supports routine consideration of OCT/IVUS guidance for PCI in severely calcified lesions to reduce 1-year target vessel failure, regardless of vessel preparation technique.

Key Findings

  • Among 2,005 patients with severely calcified lesions, IVI guidance was used in 62.1% and angiography alone in 37.9%.
  • 1-year target vessel failure was lower with IVI versus angiography (9.3% vs 13.2%; adjusted HR 0.74; 95% CI 0.56–0.97; P=0.03).
  • Benefit of IVI guidance was consistent irrespective of orbital atherectomy or balloon angioplasty vessel preparation.

Methodological Strengths

  • Large randomized trial framework with prespecified 1-year clinical endpoint (TVF).
  • Adjusted analyses and consistency across different vessel preparation strategies.

Limitations

  • Use of IVI was not randomized; potential selection and confounding biases remain.
  • Follow-up limited to 1 year; generalizability beyond severely calcified lesions requires caution.

Future Directions: Randomized allocation of IVI guidance, cost-effectiveness analyses, and extension to broader lesion subsets and longer-term outcomes.

3. Neutrophil-to-lymphocyte ratio for risk stratification in acute myocarditis across the left ventricular ejection fraction spectrum.

72Level IIICohortEuropean journal of heart failure · 2025PMID: 41098018

In a multicentre cohort of 1,150 biopsy/CMR-proven myocarditis cases, an NLR ≥4 provided prognostic discrimination comparable to traditional high-risk definitions and outperformed them in patients with preserved LVEF (AUC 0.73 vs 0.52). NLR is a simple, accessible biomarker to aid risk stratification across the LVEF spectrum.

Impact: Provides an immediately implementable, low-cost biomarker-based tool to refine risk stratification in myocarditis, addressing a gap particularly in patients with preserved LVEF.

Clinical Implications: Incorporating NLR (threshold ≥4) into initial assessment may improve triage, monitoring intensity, and follow-up planning, especially when LVEF is preserved and traditional definitions underperform.

Key Findings

  • Among 1,150 biopsy/CMR-proven cases with median 228-week follow-up, 5.2% reached death or transplant.
  • NLR had AUC 0.72 overall, comparable to complicated/high-risk definitions (AUC 0.73) and superior to fulminant classification (AUC 0.62).
  • In preserved LVEF (≥50%), NLR achieved AUC 0.73 versus 0.52 for both complicated and high-risk definitions.

Methodological Strengths

  • Large international multicentre cohort with biopsy/CMR-confirmed myocarditis.
  • Direct comparison of NLR against established high-risk definitions and stratified analysis by LVEF.

Limitations

  • Observational design with potential residual confounding and centre heterogeneity.
  • Event rates were relatively low, and external validation of the NLR threshold may be needed.

Future Directions: Prospective validation of NLR thresholds, integration with imaging/biomarker panels, and evaluation of NLR-guided management pathways.