Optimization and validation of a multi-residue method for analyzing organic UV absorbers in human urine by UHPLC-MS/MS.

Summary

The authors optimized and validated an LLE–UHPLC-MS/MS method to quantify 24 organic UV absorbers and metabolites in urine with low ng/mL LLOQs and acceptable recoveries. Applying the method to 48 healthy individuals detected 18 compounds with detection rates up to 100%, evidencing widespread exposure.

Key Findings

• Developed and validated a urine LLE–UHPLC-MS/MS assay for 24 organic UV absorbers and metabolites across multiple chemical classes.
• Achieved recoveries of 70.4–130% and LLOQs as low as 0.003–0.031 ng/mL depending on class.
• In 48 healthy adults, 18 OUVAs were detected with detection rates from 2.08% to 100%, indicating widespread exposure.

Clinical Implications

Supports population exposure assessment and informs clinicians and public health on potential endocrine and dermatologic risks from UV filter use; may guide patient counseling and policy decisions.

Limitations

• Single-population (Chinese healthy adults) cross-sectional application; not linked to health outcomes
• Matrix effects and inter-laboratory reproducibility beyond the study setup remain to be evaluated

Future Directions

Standardize inter-laboratory protocols, expand to diverse populations and longitudinal designs, and integrate exposure data with endocrine and dermatologic outcomes.