Laccase-mimicking enzymes with synergistic amplification effects on catalytic activity for ergothioneine multi-pattern logic analysis.

Summary

A laccase-mimicking catalyst (CuPH@KMO) enabled dual colorimetric/fluorescent detection of ergothioneine by inhibiting dopamine/resorcinol oxidation to azamonardine, and realized AND/INHIBIT logic-gated analysis. The method was successfully applied to cosmetics and dietary supplements, suggesting a versatile platform for quality control and smart bioanalysis.

Key Findings

• CuPH@KMO showed enhanced catalytic efficiency and broader pH tolerance compared with typical laccase activity.
• Dopamine and resorcinol were oxidized to blue fluorescent azamonardine (Ex=478 nm, Abs=420 nm), enabling dual readouts.
• EGT inhibited the CuPH@KMO-catalyzed reactions, forming the basis for colorimetric and fluorescence detection.
• An AND/INHIBIT logic gate architecture was constructed and the method was successfully applied to cosmetics and dietary supplements.

Clinical Implications

No immediate change to bedside care; however, this platform can improve quality control of cosmetic products and supplements containing ergothioneine, supporting safety, label accuracy, and regulatory compliance.

Limitations

• Analytical performance metrics (e.g., limits of detection, interference panels) are not detailed in the abstract
• Clinical relevance is indirect and broader validation across diverse cosmetic formulations is needed

Future Directions

Quantify detection limits, robustness to interferents, and standardize protocols for regulatory QC; explore on-chip integration and extension to other antioxidant analytes.