Single-cell RNA-seq analysis identifies the atlas of lymph fluid and reveals a sepsis-related T cell subset.

Summary

Using scRNA-seq of rat lymph fluid, the authors built a comprehensive immune cell atlas and identified a distinct CD4+ T-cell subset associated with sepsis. This delineates lymph-resident immune heterogeneity and suggests new mechanistic targets for sepsis immunomodulation.

Key Findings

• Generated a single-cell atlas of immune cells from rat lymph fluid using scRNA-seq.
• Identified a unique CD4+ T-cell subset associated with sepsis.
• Revealed lymph-resident immune heterogeneity relevant to systemic inflammatory responses.

Clinical Implications

Although preclinical, the defined T-cell subset may serve as a biomarker or therapeutic target guiding host-directed sepsis therapies after human validation.

Limitations

• Preclinical rat model; human validation is required
• Abstract truncation limits detail on validation and functional assays

Future Directions

Validate the sepsis-related T-cell subset in human lymph/blood, define functional roles, and test as biomarker/target in interventional studies.