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KVX-053, a protein tyrosine phosphatase 4A3 inhibitor, ameliorates SARS-CoV-2 spike protein subunit 1-induced acute lung injury in mice.

The Journal of pharmacology and experimental therapeutics2025-02-19PubMed
Total: 73.0Innovation: 8Impact: 7Rigor: 7Citation: 7

Summary

In a K18-hACE2 mouse model of SARS-CoV-2 spike S1-induced ALI, the selective allosteric PTP4A3 inhibitor KVX-053 reduced inflammation, vascular leak, structural injury, and lung dysfunction. This is the first evidence implicating PTP4A3 as a therapeutic target in COVID-19-associated ALI/ARDS.

Key Findings

  • SARS-CoV-2 spike S1 instillation in K18-hACE2 mice induced pulmonary/systemic inflammation, alveolar leak, cytokine overexpression, structural injury, and dysfunction.
  • KVX-053, a selective allosteric PTP4A3 inhibitor, ameliorated these injury phenotypes.
  • Provides first evidence implicating PTP4A3 in SARS-CoV-2-mediated ALI pathogenesis.

Clinical Implications

PTP4A3 inhibition may complement antivirals by mitigating host-driven vascular leak and inflammation, offering a host-targeted adjunctive strategy for viral ARDS.

Why It Matters

Identifies a new, druggable host pathway (PTP4A3) with translational relevance to COVID-19 and potentially other viral ARDS entities.

Limitations

  • Spike S1 protein instillation model does not recapitulate full viral infection dynamics.
  • Lack of survival, dose–response, and pharmacokinetic data in the abstract.

Future Directions

Test KVX-053 in live-virus models and diverse ARDS etiologies; define PK/PD and safety; dissect PTP4A3 signaling nodes in pulmonary endothelium/epithelium; explore combination with antivirals.

Study Information

Study Type
Case-control
Research Domain
Treatment
Evidence Level
V - Preclinical animal study demonstrating target engagement and phenotypic rescue.
Study Design
OTHER