Daily Cosmetic Research Analysis

BACKGROUND: Cholangiocarcinoma presents a global health challenge due to its increasing incidence and poor prognosis, primarily resulting from delayed diagnosis. There is an urgent need for a reliable biomarker to enhance early detection. MATERIALS AND METHODS: Patients with cholangiocarcinoma were enrolled into three cohorts: a discovery set (n = 6), a verification set (n = 34), and a validation set (n = 146), for seeking potential biomarkers, while patients with gallstones served as controls. Three cholangiocarcinoma transcriptome datasets from the gene expression omnibus (GEO) were analyzed. Techniques employed included liquid chromatography-tandem mass spectrometry (LC-MS/MS), multiple reaction monitoring, and enzyme-linked immunosorbent assay. RESULTS: Using a discovery set, bile proteome profiling identified 57 upregulated proteins that were either unique to cholangiocarcinoma or exhibited ≥ 2-fold changes compared to controls. The GEO transcriptome datasets yielded 48 upregulated genes consistently expressed in cholangiocarcinoma. POSTN (periostin) emerged as a viable biomarker by intersecting these two omics analyses. In the verification set, bile and plasma POSTN levels in cholangiocarcinoma were 4.7-fold and 2.1-fold higher, respectively, compared to controls. In the validation set, the sensitivity, specificity, and AUC of plasma POSTN for diagnosing cholangiocarcinoma were 78%, 85%, and 0.86, respectively, compared to 67%, 90%, and 0.86 for CA19.9. The combination of plasma POSTN and CA19.9 improved these metrics to 87%, 91%, and 0.94, respectively. Protein interactome analysis demonstrated that POSTN was predominantly connected to structural proteins of extracellular matrix (ECM). Patients with higher plasma POSTN levels exhibited higher expression of transcriptional regulators of epithelia-mesenchymal transition (EMT) and worse overall survival compared with those with lower levels. CONCLUSIONS: Plasma POSTN, derived from the bile proteome, demonstrates both comparable and complementary efficacy to CA19.9, emerging as a promising biomarker for diagnosing cholangiocarcinoma. Beyond its diagnostic capability, POSTN's role in extracellular matrix interactions and EMT regulation highlights its prognostic potential.

BACKGROUND: Craniofacial defects from cancer surgery led to functional, aesthetic, and psychological challenges. Rehabilitation with craniofacial implants addresses these issues by improving prosthesis retention through osseointegration and providing predictable cosmetic results. However, maintaining a healthy transcutaneous region is essential for implant longevity. OBJECTIVE: Evaluation of the microbial community and host response around extraoral implants. METHODS: In an intrasubject control study design, 12 cancer patients who had undergone oculofacial rehabilitation with implant-supported prostheses were included. Biofilm and peri-implant fluid samples were collected from the transcutaneous region of healthy and diseased implants. Microbiome profiling was conducted through DNA sequencing of the V3-V4 region of the 16S rRNA gene, and proteome analysis was performed using liquid chromatography-tandem mass spectrometry. RESULTS: Differentially abundant species were observed, with Streptococcus intermedius being the most abundant in diseased areas, followed by Corynebacterium diphtheriae and Prevotella bivia. Metaproteomic analysis revealed distinct protein expression patterns between the groups, with increased activation of proinflammatory responses and inactivation of anti-inflammatory responses in the diseased group. CONCLUSION: The study demonstrated an increased abundance of pathogenic bacterial community accompanied by an imbalanced immune response, thereby highlighting host-microbial factors that can influence the success of osseointegration and facial rehabilitation.

BACKGROUND: Breast augmentation is the most common cosmetic surgery worldwide. Despite recent approval for aesthetic use, adipose tissue autografting remains underutilized compared to breast implants, the standard for hypoplasia correction. OBJECTIVES: This study aims to compare patient satisfaction and quality of life following breast augmentation with implants or lipofilling, using the BREAST-Q questionnaire. METHODS: From July 2017 to 2022, patients from our Plastic, Reconstructive, and Aesthetic Surgery Department who underwent breast augmentation with implants or lipofillin were included. The primary endpoint was patient satisfaction and quality of life, assessed using the BREAST-Q questionnaire at least six months postoperatively. RESULTS: Among 119 patients (87 implants, 32 lipofilling), BREAST-Q scores showed no significant difference in breast satisfaction (71/100 vs. 69/100). Psychosocial and sexual well-being scores were similar between groups, while physical well-being was higher in the lipofilling group (96/100 vs. 87/100). Major complications occurred in 3.4% of the implant group, whereas none were observed in the lipofilling group. CONCLUSION: Adipose tissue autografting is an effective technique, providing comparable patient satisfaction to breast implants while offering better physical well-being. It is a viable alternative to implants, particularly for patients seeking moderate breast augmentation or avoiding foreign materials, provided they have sufficient fat reserves. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .