Daily Cosmetic Research Analysis

Recent research has underscored the significant role of skin fungi in human health and disease. The advent of next-generation sequencing has facilitated the identification of previously unrecognized fungi; however, a standardized sampling method for the skin mycobiome has yet to be established. Given that fungi are distributed across all layers of the skin, this study aimed to establish and compare the fungal profiles obtained from paired skin swabs (skin surface) and tissue specimens (skin dermis), utilizing ITS rRNA gene sequencing. Fungal diversity and composition varied between swabs and tissue specimens from the same individual. Tissue specimens exhibited greater fungal diversity relative to skin swabs, as indicated by the Chao1 estimator (P=0.028) and Shannon index (P=0.0136). Notably, Malassezia species was more abundant in swabs (P=0.002), whereas Trichoderma species was more prevalent in tissue samples (P=0.0001). Compared to swabs, tissue sampling also demonstrated superior sensitivity in detecting non-Malassezia species. These findings suggest that skin swabs and tissue specimens provide different views of the skin mycobiome: Swabs are effective for studies targeting surface-dwelling fungi, while tissue sampling remains necessary when investigating deeper skin fungi and potential systemic infections. Future research should carefully consider appropriate sampling method based on target fungal location to mitigate procedural artifacts.Level of Evidence III This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

PURPOSE: Intense pulsed light (IPL) treatment may cause transient erythema, dryness, and barrier dysfunction, highlighting the need for effective postprocedural care. This study aimed to assess the efficacy and safety of a fibronectin-containing skincare regimen in restoring skin barrier function and relieving these IPL-related manifestations. PATIENTS AND METHODS: In this 28-day, split-face study, 32 healthy female participants undergoing IPL treatment applied a fibronectin-containing serum to one side of the face and a control serum to the opposite side. Skin hydration, transepidermal water loss (TEWL), erythema, and radiance were evaluated at baseline, immediately post-IPL, and on days 3, 7, and 28. RESULTS: Compared to the control, the fibronectin-treated side showed significantly greater improvements in skin hydration (P < 0.01) and significantly lower TEWL on days 3 (P < 0.05), 7 (P < 0.01), and 28 (P < 0.01). By days 7 and 28, skin radiance and erythema also improved more significantly (P < 0.01). Both dermatologist and participant assessments confirmed the superior efficacy of the fibronectin serum. No adverse events were rePorted. CONCLUSION: Post-IPL aPPlication of a fibronectin-containing serum effectively imProves skin hydration, reduces barrier dysfunction and erythema, and enhances radiance with good tolerability. These findings suPPort its Potential utility in Post-IPL skincare regimens.

INTRODUCTION: Tuberous breast is a congenital breast deformity that can have a significant impact. Most therapeutic algorithms involve glandular remodeling, with or without the addition of a breast implant. The increased use of fat grafting in breast surgery has introduced a new tool in the surgical arsenal. We conducted a retrospective study. The primary objective was to evaluate our surgical technique. The secondary objective was to assess patient satisfaction in a larger cohort than previously studied. MATERIALS AND METHODS: This retrospective study included patients operated for unilateral or bilateral breast deformities. The fat grafting technique was offered to patients without any personal or familial history of breast cancer and with a mammogram performed within six months rated ACR 1 or 2. Patient satisfaction was retrospectively assessed at a minimum of 6 months post-op using the Breast-Q® augmentation module and an analog scale rated from 1 to 4. RESULTS AND DISCUSSION: Statistical analysis was conducted on the 81 patients (103 breasts) included in the study. The median was two interventions. The only complication found was cystic fat necrosis cysts in 3% of breasts (n=3), with only one requiring surgical fragmentation. Patient evaluation was not correlated with the stage of the tuberous breast. According to our satisfaction scale, 90.9% of patients were satisfied or very satisfied with the overall appearance of their breasts. CONCLUSION: Fat grafting should not be considered merely as a volume augmentation but as a true reactivation of breast growth, akin to a localized pubertal restart of an underdeveloped breast. The quality of results obtained, the very high level of patient satisfaction, the very low complication rate, and the definitive nature of the reconstruction have made this technique our Gold Standard for managing tuberous breasts. LEVEL OF EVIDENCE III: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .