Daily Cosmetic Research Analysis

Roses are economically important ornamental plants, with widespread applications in the cut flowers, garden and cosmetics industries. The genomic evolution and diversity of the subgenus Rosa remain understudied, limiting exploitation of its diversity in breeding. Here we assembled genomes of 23 accessions, comprising 51 haplotypes that capture the subgenus's high genetic diversity. Extensive introgression across accessions from different sections highlights crossbreeding potential. Pangenome analysis revealed 1,801,537 structural variations, providing insights into the genetic basis and key regulators controlling key traits such as continuous flowering, petal number and discoloration. A key finding was the identification of a CCD4 homolog as the main regulator of petal discoloration. Additional subgenomic analysis of the allopolyploid Rosa gallica and the triploid Rosa hybrida 'La France', two important breeding materials of modern roses, revealed their hybrid origins. Overall, this study advances understanding of rose genomics and provides valuable resources for future breeding and trait improvement.

BACKGROUND: The optimal method of wound closure after ileostomy reversal remains uncertain. Pursestring approximation reduces surgical site infections but requires prolonged wound care. Combining negative pressure wound therapy with primary linear closure may offer a more convenient approach while maintaining acceptable infection rates. OBJECTIVE: To determine whether primary linear closure with negative pressure wound therapy is noninferior to pursestring approximation in preventing surgical site infection after ileostomy reversal. DESIGN: Prospective, non-blinded, multi-institutional, non-inferiority randomized controlled trial. SETTINGS: Community tertiary hospital and academic medical center. PATIENTS: Adults undergoing elective ileostomy reversal between October 2018 and March 2024. INTERVENTION: Participants were randomized to undergo either primary linear closure with negative pressure wound therapy or pursestring approximation for skin closure after ileostomy reversal. MAIN OUTCOME MEASURES: The primary outcome was the occurrence of surgical site infection, with a non-inferiority margin set at 16%. Secondary outcomes included time to wound healing and scar appearance using the validated Patient and Observer Scar Assessment Scale. RESULTS: One hundred twelve patients completed the study, with 61 in the negative pressure wound therapy arm and 51 in the pursestring approximation arm. Primary linear closure- negative pressure wound therapy was noninferior to pursestring approximation arm in terms of SSI: 7% and 2% respectively with an absolute risk difference of 5% (95% CI: 2.5% to 12.5%). Early wound healing at 2 weeks was achieved in 77% vs 23.5% of patients, respectively (p < 0.001, GEE analysis). All wounds healed by 6 weeks in both groups. No negative pressure wound therapy device malfunctions occurred. LIMITATIONS: Nonblinded study limited to 2 institutions. CONCLUSIONS: Ileostomy reversal by Primary linear closure- negative pressure wound therapy is noninferior to pursestring approximation in terms of the occurrence of surgical site infections while achieving significantly faster wound healing. The method is safe, convenient and cosmetically acceptable. (See Video Abstract)Trial Registration: Advocate Aurora Health IRB, IRB# 22.034 (7082).

Next-generation risk assessment (NGRA) aims to enable transparent, reproducible chemical safety assessments based on human-relevant, animal-free new approach methodologies (NAMs). The Alternative Safety Profiling Algorithm (ASPA) was developed within the ASPIS cluster to provide an algorithmic workflow that structures problem formulation, evidence integration, and decision-making across three main pillars-hazard, ADME (toxicokinetics), and exposure. To refine ASPA, a stakeholder workshop was organized. Four breakout groups systematically reviewed corresponding workflow sections, identifying strengths, conceptual gaps, and opportunities for harmonization. Across groups, participants endorsed ASPA's modular, technology-neutral nature and its focus on standardizing processes rather than prescribing specific test batteries. The hazard pillar discussions emphasized a sensitive, hypothesis-generating Tier 1, complemented by a specific, mechanistic Tier 2, capable of deriving points of departure (PoDs). ADME experts supported a physiology-based kinetic (PBK) modelling strategy, advancing from generic towards more complex models, using mechanistic information and experimental data. The exposure group proposed refinements for transparent, tiered exposure modelling, with emphasis on realistic worst-case scenarios and explicit uncertainty communication. Cross-pillar discussions highlighted the importance of feedback loops between all pillars, and the documentation of decision points to achieve consistency and defensibility. The workshop outcomes informed three parallel developments: (i) algorithmic refinement and re-design toward the next ASPA version, (ii) the creation of detailed guidance for each building block, and (iii) the establishment of practical case studies to demonstrate workflow implementation. This report already contains a first case study (developmental neurotoxicity assessment of desnitro-imidacloprid). These advances increase the operability, transparency, and regulatory readiness of ASPA. Next-generation risk assessment (NGRA) aims to make chemical safety testing more human-relevant without using animals. The Alternative Safety Profiling Algorithm (ASPA) offers a structured way to combine all required data. These include information on chemical hazards, predictions on how chemicals move through the body (ADME), and estimates of exposure levels. A stakeholder workshop reviewed and improved the ASPA workflow and the associated software platform. Participants stressed the importance of the tool to make NGRA transparent and reproducible between different groups of evaluators. They suggested refinements for each part, such as clearer exposure estimates that include uncertainty. They also stressed the need for feedback between the different main modules of ASPA. An example of an ASPA evaluation (desnitroimidacloprid) was prepared for illustration of all process steps.