Method Development for Determining β-Nicotinamide Mononucleotide (NMN) in Cosmetics Using m-PFC-HPLC.
Total: 70.0Innovation: 7Impact: 7Rigor: 7Citation: 7
Summary
An m-PFC (MWCNT)-assisted HPLC method was developed and validated to quantify NMN in facial mask essence, emulsions, and creams, achieving high recovery and low RSD with LOQ at 5.0 mg/kg. Application to seven commercial products revealed that some items labeled as containing NMN had no detectable NMN.
Key Findings
- Established an m-PFC (MWCNT)-HPLC method with linearity 5.0–500 μg/mL and LOQ 5.0 mg/kg across three cosmetic matrices.
- Precision RSD <3% and recovery 93.9–109.4% (RSD <3.7%) indicate high accuracy and repeatability.
- Applied to seven e-commerce cosmetics, detecting absence of NMN in some products claiming NMN.
Clinical Implications
Regulatory labs and manufacturers can implement this workflow to verify NMN content, protect consumers, and standardize label claims.
Why It Matters
Provides a reproducible QC method for NMN in complex cosmetic matrices and uncovers potential mislabeling, directly informing regulatory oversight.
Limitations
- Evaluated only three matrix types; broader applicability to other formulations needs confirmation
- Method targets NMN specifically; related metabolites or precursors were not assessed
Future Directions
Validate across additional cosmetic matrices and harmonize with regulatory standards; extend to multiplex detection of related NAD+ precursors.
Study Information
- Study Type
- Case series
- Research Domain
- Diagnosis
- Evidence Level
- V - Analytical method development and validation with application to market samples
- Study Design
- OTHER