A Dual-Response DNA Origami Platform for Imaging and Treatment of Sepsis-Associated Acute Kidney Injury.
Summary
A DNA origami theranostic platform responds to elevated miR-21 in SA-AKI, enabling dual fluorescence and photoacoustic imaging while scavenging ROS and delivering LL-37 for antimicrobial activity. In preclinical models, the integrated approach improved survival by 80%, showcasing precision nanomedicine for sepsis-related organ injury.
Key Findings
- miR-21-triggered strand displacement in DNA origami restores Cy5 fluorescence, enabling real-time SA-AKI detection with dual fluorescence and photoacoustic imaging.
- DNA origami exhibits ROS-scavenging properties and, when conjugated with LL-37, provides bactericidal activity.
- Theranostic integration improved survival by 80% in SA-AKI preclinical models.
Clinical Implications
If translated, such theranostics could enable earlier identification of SA-AKI and timely antimicrobial/antioxidant interventions, potentially improving outcomes beyond current supportive care.
Why It Matters
Introduces a programmable nanoplatform that unites early detection and targeted therapy in SA-AKI, a major contributor to sepsis morbidity and mortality.
Limitations
- Preclinical models; human pharmacokinetics, biodistribution, and safety remain unknown.
- Complex manufacturing and regulatory pathways for DNA nanostructures.
Future Directions
Scale up GMP-compatible manufacturing, evaluate safety/tox in large animals, and design early-phase trials for high-risk sepsis populations with emerging AKI.
Study Information
- Study Type
- Basic/Mechanistic research
- Research Domain
- Diagnosis/Treatment
- Evidence Level
- V - Preclinical theranostic proof-of-concept with survival outcomes in animal models
- Study Design
- OTHER