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Daily Cosmetic Research Analysis

3 papers

Three studies shape cosmetic and aesthetic dermatology today: a multi-laboratory validation of the KeraSkin Phototoxicity Assay supports its inclusion in OECD TG 498 and strengthens non-animal safety testing; a randomized split-face trial shows CE Ferulic serum reduces erythema and speeds recovery after nonablative fractional laser; and an investigator-blinded split-face non-inferiority trial finds adipose MSC-derived exosomes comparable to platelet-rich plasma for photoaged skin.

Summary

Three studies shape cosmetic and aesthetic dermatology today: a multi-laboratory validation of the KeraSkin Phototoxicity Assay supports its inclusion in OECD TG 498 and strengthens non-animal safety testing; a randomized split-face trial shows CE Ferulic serum reduces erythema and speeds recovery after nonablative fractional laser; and an investigator-blinded split-face non-inferiority trial finds adipose MSC-derived exosomes comparable to platelet-rich plasma for photoaged skin.

Research Themes

  • Non-animal phototoxicity safety testing
  • Post-laser cosmeceutical care and recovery
  • Regenerative aesthetics: exosomes versus PRP

Selected Articles

1. KoCVAM-led validation of KeraSkin™ Phototoxicity Assay for inclusion in OECD TG 498.

74Level IVCase seriesALTEX · 2025PMID: 40417838

A five-laboratory validation demonstrated that the KeraSkin reconstructed human epidermis phototoxicity assay achieves 97.6–100% predictive performance across reference and test chemical sets, meeting OECD TG 498 inclusion criteria. The assay enables robust, animal-free phototoxicity assessment for cosmetics and drugs.

Impact: Regulatory-grade validation of an animal-free phototoxicity assay directly supports safety assessment frameworks and reduces animal use while maintaining high predictive accuracy.

Clinical Implications: While not a clinical study, adoption of this assay can improve pre-market safety screening of topical agents, potentially reducing phototoxic adverse events in real-world use and streamlining regulatory submissions.

Key Findings

  • Within-lab reproducibility was 91.7–100% and between-lab reproducibility was 100% for 12 reference chemicals across all labs.
  • For 12 reference chemicals over 144 runs, sensitivity was 100% (72/72), specificity 98.6% (71/72), and accuracy 99.3% (143/144).
  • For 32 test chemicals over 164 runs, sensitivity, specificity, and accuracy were each 97.6% (80/82, 80/82, 160/164).
  • The assay fulfills requirements for inclusion as a ‘me-too’ method in OECD TG 498.

Methodological Strengths

  • Multi-laboratory validation aligned with OECD Performance Standard No. 356
  • High reproducibility and balanced sensitivity/specificity across reference and test sets

Limitations

  • Designated as a ‘me-too’ method; innovation is incremental rather than first-in-class
  • Validation focused on selected reference/test chemicals; generalizability to all photoreactive chemistries may require further testing

Future Directions: Broaden chemical applicability domains, compare head-to-head with existing OECD TG 498 methods, and develop decision trees integrating RhE phototoxicity data into risk assessment frameworks.

2. A Randomized, Investigator-Blinded, Split-Face, Controlled Trial Assessing Efficacy and Satisfaction of CE Ferulic Serum Following Nonablative Fractional Fraxel Laser Treatment for Photoaging Skin in Chinese Population.

72.5Level IRCTJournal of cosmetic dermatology · 2025PMID: 40414817

In a randomized, investigator-blinded split-face trial (n=50), CE Ferulic serum applied immediately after nonablative fractional laser and for 7 days reduced erythema progression versus saline and improved erythema/melanin indices, hydration, satisfaction, and pain.

Impact: Provides randomized evidence to optimize post-laser cosmeceutical care, addressing common downtime symptoms and potentially improving patient experience and recovery.

Clinical Implications: Consider CE Ferulic as a post-NAFL regimen to attenuate erythema and support barrier function during the first week, with counseling on expected benefits and monitoring for irritation.

Key Findings

  • Day-7 erythema score change was significantly lower on the CEF-treated side versus saline (0.04±0.40 vs 0.18±0.48; p=0.011).
  • CEF improved erythema index, melanin index, and skin hydration compared with saline.
  • Patient-reported outcomes favored CEF with higher overall satisfaction and less post-procedure pain.

Methodological Strengths

  • Randomized, investigator-blinded, split-face controlled design
  • Prospectively registered trial with objective biophysical endpoints (EI, MI, hydration)

Limitations

  • Short follow-up limited to 7 days and single-population (predominantly young female) sample
  • Single comparator (saline) without active control; single-center design

Future Directions: Evaluate longer-term outcomes, diverse skin phototypes, comparisons with other post-laser actives, and cost-effectiveness.

3. Adipose Mesenchymal Stem Cell-Derived Exosomes Versus Platelet-Rich Plasma Treatment for Photoaged Facial Skin: An Investigator-Blinded, Split-Face, Non-Inferiority Trial.

69Level IIRCTJournal of cosmetic dermatology · 2025PMID: 40414798

In an investigator-blinded split-face non-inferiority trial with three RF microneedling sessions, topical adipose MSC-derived exosomes matched PRP in improving wrinkles, dyschromia, erythema, texture, and overall appearance, with histology confirming increased collagen I and glycosaminoglycans in both arms.

Impact: Offers first head-to-head comparative evidence positioning exosomes as a practical alternative to PRP for photoaged skin, with implications for patient preference and clinic workflow.

Clinical Implications: Topical exosomes can be considered for patients who prefer to avoid venipuncture or when PRP processing is impractical, potentially reducing visit time while achieving comparable outcomes.

Key Findings

  • Exosomes and PRP produced equivalent improvements in wrinkles, dyschromia, erythema, texture, and overall appearance.
  • Histology showed increased collagen I and glycosaminoglycans in both treatment arms without significant differences.
  • Three sessions of RF microneedling were combined with split-face application (PRP vs topical exosomes) under investigator blinding.

Methodological Strengths

  • Investigator-blinded split-face design controls for inter-individual variability
  • Inclusion of histologic endpoints (collagen I and GAG) to corroborate clinical findings

Limitations

  • Sample size and detailed quantitative outcomes not specified in the abstract
  • Follow-up duration and trial registration status not reported; non-inferiority margins not described

Future Directions: Conduct larger, registered non-inferiority RCTs with predefined margins, longer follow-up, standardized exosome preparations, and safety monitoring.